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caspase-3 (A20) Antibody DB008 0.200 mg/ml $190.00

Datasheet 
Datasheet 

For technical service please call (800) 595 1994
Product Info
Background Caspases is a family of mammalian proteases that regulate many of the morphological and biochemical features of apoptosis. The currently identified caspases can be divided into three groups: apoptotic initiators, apoptotic executioners, and inflammatory mediators (1,2). Caspase-2, 8, and 9 have been identified as apoptotic initiators (3,4). Caspase-2 (also designated Nedd-2/ICH-1) can be alternatively spliced to produce two distinct Caspase-2 mRNA species. One mRNA species encodes a protein of 435 amino acids in length and the mRNA splice variant encodes a 312 amino acid protein. The larger protein, Caspase-2L, in an over expressed state, leads to programmed cell death. Conversely the over expression of Caspase-2S protein, a truncated version of Caspase-2L, has been shown to suppress cell death induced by serum deprivation (5). The executioner caspases include caspase-3, 6 and 7. Of these three proteases Caspase-3 is considered to be the essential for the chromatin margination, DNA fragmentation, and nuclear collapse during apoptosis (6). Caspase-1 (also known as ICE) is considered to be a mediator of the inflammatory response by converting the inactive precursor of interleukin-1b to the 17 kDa proinflammatory cytokine, IL-1b (7,8). 
*Product Citations* 1.) Kim HJ, Kang SY, Kim SJ, Kim SH, Kim TY. 2004. Potentiation of UVB-induced apoptosis by novel phytosphingosine derivative, tetraacetyl phytosphingosine in HaCaT cell and mouse skin. Apoptosis. Jul;9(4):449-56. 
Origin Caspase-3 (also known as CPP32 ) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the amino terminus of human caspase-3 precursor. 
Product Details Each vial contains 200 g/ml of affinity purified rabbit IgG, caspase-3 DB008 (A20), in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Competition Studies A blocking peptide is also available, DB008P, for use in competition studies. Each vial contains 0.100 mg of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 mg BSA. 
Form 200 g/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Immunogen Synthetic peptide mapping to the amino terminal domain of human caspase-3 prescursor. 
Specificity Human caspase-3 precursor (also designated CPP32) 
Use Western blotting, immunoprecipitation, and immunohistochemistry 
Storage Store this product at 4 C, do not freeze. The product is stable for one year from the date of shipment. 
References 1. Edadah BA, Faden AI. 2000. Caspase pathways, neuronal apoptosis, and CNS injury. Jounal of Neurotauma 17(10): 811-829
2. Salvesen GS, Dixit VM. 1999. Caspase activation: the induced-proximity model. Proc Natl Acad Sci USA 96(20): 10964-10967
3. Zhang W, He Q, Chan LL, Zhou F, El Naghy M, Thompson EB, Ansari NH. 2001. Involvment of caspases in 4-hydroxy-alkenal-induced apoptosis in human leukemic cells. Free Radic Biol Med 30(6): 699-706
4. Kruidering M, Evan GI. 2000. Caspase-8 in apoptosis: the beginning of ?the end? ? IUBMB 50(2): 85-90
5. Wang L, Miura M, Bergeron L, Zhu H, Yuan J. 1994. Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death. Cell 78(5): 739-750
6. Slee EA, Adrain C, Martin SJ. 2001. Executioner caspase-3, -6, and ?7 perform distinct, non-redundant roles during the demolition phase of apoptosis. J Biol Chem 276(10): 7320-7326
7. Kostura MJ, Tocci MJ, Limjuco G, Chin J, Cameron P, Hillman AG, Chartrain NA, Schmidt JA. 1989. Identification of a monocyte specific pre-interleukin 1b convertase activity. Proc Natl Acad Sci USA 86(14): 5227-5231
8. Wilson KP, Black JA, Thompson JA, Kim EE, Griffith JP, Navia MA, Murcko MA, Chambers SP, Aldape RA, Raybuck SA, Livingston DJ. 1994. Structure and mechanism of interleukin-1b converting enzyme. Nature 370(6487): 251-252
 

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Protein Kinases| Secondary Antibodies| Signaling Intermediates| Transcription Regulation