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GFP (C19) Antibody DB064 0.200 mg/ml $190.00

Datasheet 
Datasheet 

For technical service please call (800) 595 1994
Product Info
Background Green fluorescent protein was originally cloned from the cnidarian, Aequorea victoria. This exceptional protein absorbs blue light (maximally at 395 nm) and emits green light (peak at 509 nm) without the requirement of exogenous substrates and cofactors (1). These unique qualities allow GFP to be used to monitor gene expression and protein localization in vivo. Several mutant forms of GFP have been developed which fluoresce more intensely and have shifted excitation maxima when compared to the wild type GFP, making them useful for FACS, fluorescence microscopy, and double-labeling applications (2,3). 
Origin GFP (C19) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping near the carboxy terminus of Aequorea victoria GFP. 
Product Details Each vial contains 200 g/ml of affinity purified rabbit IgG, GFP (C19) DB064, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Competition Studies A blocking peptide is also available, DB064P, for use in competition studies. Each vial contains 0.100 mg of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 mg BSA. 
Form Each vial contains 200 g/ml of affinity purified rabbit IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Immunogen Synthetic peptide mapping near the carboxy terminus of Aequorea victoria GFP. 
Specificity GFP (C19) DB064 recognizes all variants of recombinant Aequorea green-fluorescent protein 
Use DB064 is recommended for use by western blotting and immunoprecipitation. 
Storage Store this product at 4 C, do not freeze. The product is stable for one year from the date of shipment. 
References 1. Chalfie M, Tu Y., Euskirchen G., Ward W.W., Prasher D.C. 1994. Green Fluorescent Protein as a Marker for Gene Expression. Science 263: 802-805.
2. Cormack B.P., Valdivia R.H., and Falkow S. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38.
3. Rizzuto R., Brini M., De Giorgi F., Rossi R., Heim R., Tsien R.Y., and Pozzan T. 1996. Double labelling of the subcellular structures with organelle-targeted GFP mutants in vivo. Curr.Biol. 6:183-188.
 

For Technical service please call +1-800-595-1994
Delta Biolabs+1-408-846-6650 • fax:+1-408-846-6645 • Copyright © 2001.Allrights reserved.
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