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PKC (MC5) Antibody DB107 0.100 mg/ml $275.00

Datasheet 
Datasheet 

For technical service please call (800) 595 1994
Product Info
Background To date at least 12 different protein kinase C (PKC) isoforms have been identified that possess distinct differences in structure, substrate requirement, expression and localization (1). The PKC family of serine/threonine protein kinases is a ubiquitous group involved in complex intracellular signaling processes that are mediated though phospholipid hydrolysis (2). The general structure of a PKC molecule consists of a catalytic and a regulatory domain; composed of a number of conserved regions interspersed with variable domains. The PKC isoforms have been divided into three groups, conventional PKC isoforms (?, ?I, ?II, and ?), novel PKC isoforms (?, ?, ?, ?, and ?), and atypical PKC isoforms (?, and ?/?). The more recently identified PKC? shows the most sequence homology to PKC?. The conventional PKC isoforms require calcium and diacylglycerol (DAG) for activation, the novel isoforms require DAG, and the atypical PKC isoforms require neither calcium nor DAG (3). 
Origin PKC (MC5) is provided as a mouse monoclonal IgG2a derived from the fusion of X-63 myeloma cells with spleen cells from a BALB/c mouse immunized with protein kinase C purified from bovine brain. 
Product Details Each vial contains 100 g/ml of mouse monoclonal IgG2a PKC (MC5) DB107, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Form 100 g/ml of mouse monoclonal IgG2a in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Immunogen Protein kinase C purified from bovine brain. 
Specificity PKC (MC5) DB107 reacts with PKC alpha, beta, and gamma of mouse, rat and human origin. 
Use Western blotting and immunoprecipitation. Western blotting starting dilution: 1:200. Will recognize alpha, beta and gamma PKC. 
Storage Store this product at 4 C, do not freeze. The product is stable for one year from the date of shipment. 
References 1. Way KJ, Chou E, King GL. 2000. Identification of PKC-isoform-specific biological actions using pharmacological approaches. Trends Pharmacol Sci. 21(5): 181-187.
2. Liu WS, Heckman CA. 1998. The sevenfold way of PKC regulation. Cell Signal. 10(8): 529-542.
3. Gschwendt M. 1999. Protein kinase C delta. Eur J Biochem. 259(3): 555-564.
 

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