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IkB-alpha (C21) Antibody DB075 0.200 mg/ml $190.00

Datasheet 
Datasheet 

For technical service please call (800) 595 1994
Product Info
Background The NFkB family of transcription factors is sequestered in the cytoplasm by IkB inhibitory proteins (1&2).IkB-alpha and IkB-beta are the two members of this family most often cited for their regulatory function of NFkB directed gene expression. The interaction of NFkB dimers with IkB proteins keeps the complex in the cytoplasm, but upon cellular stimulation the IkB proteins are phosphorylated and subsequently degraded (1&3). The free NFkB can now enter the nucleus and induce gene expression of proteins critical for cellular proliferation, differentation, or apoptosis (1-4). 
Origin IkB-alpha (C21) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminal domain of human IkB-alpha. 
Product Details Each vial contains 200 g/ml of affinity purified rabbit IgG, IkB-alpha (C21) DB075, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Competition Studies A blocking peptide is also available, DB075P, for use in competition studies. Each vial contains 0.100 mg of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 mg BSA. 
Form 200 g/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Immunogen Synthetic peptide mapping to the carboxy terminal domain of human IkB-alpha. 
Specificity IB- (C21) is recommended to detect mouse, rat and human IkB-alpha. 
Use Western blotting, immunoprecipitation, and immunohistochemistry (including paraffin-embedded tissue). Recommended western blotting starting dilution 1:200. 
Storage Store this product at 4º C, do not freeze. The product is stable for one year from the date of shipment. 
References 1. Schmitz ML, Baeuerle PA. 1995. Multi-step activation of NF-kappa B/Rel transcription factors. Immunobiology 193(2-4):116-127.
2. Tran K, Merika M, Thanos D. 1997. Distinct functional properties of IkappaB alpha and IkappaB beta. Mol Cell Biol 17(9):5386-5399.
3. Tanaka K, Kawakami T, Tateishi K, Yashiroda H, Chiba T. 2001. Control of IkappaBalpha proteolysis by the ubiquitin-proteasome pathway. Biochimie 83(3-4):351-356.
4. Chen CG, Malliaros J, Katerelos M, d?Apice AJ, Pearse MJ. Inhibition of NF-kappaB activation by a dominant-negative mutant of IkappaBalpha. Mol. Immunology 33(1):57-61.
 

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Apoptosis/Tumor Suppressor Genes| Cell Adhesion| Cell Cycle| Controls| Fusion Protein/Epitope Tags| Growth Factors| Membrane Receptors
Protein Kinases| Secondary Antibodies| Signaling Intermediates| Transcription Regulation