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beta-Actin (A11) Antibody DB070 0.200 mg/ml $190.00

Datasheet 

For technical service please call (800) 595 1994
Product Info
Background All eukaryotic cell types express actin. The regulation of the actin cytoskeleton is tightly linked to vital biological properties such as polarity, motility, cell-cell contact, exocytosis and proliferation (1&2). While lower eukaryotes such as yeast, have only one actin gene; higher eukaryotes have several isoforms encoded by a family of genes (3&4). At least six types of actin are present in mammalian tissues and fall into three classes (5). Alpha-actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues (6). 
*Product Citations* 1.) Hoffrogge R, Mikkat S, Scharf C, Beyer S, Christoph H, Pahnke J, Mix E, Berth M, Uhrmacher A, Zubrzycki IZ, Miljan E, Volker U, Rolfs A. 2006. 2-DE proteome analysis of a proliferating and differentiating human neuronal stem cell line (ReNcell VM). Proteomics. Mar;6(6):1833-47.
2.) Dogan S, Hu X, Zhang Y, Maihle NJ, Grande JP, Cleary MP. 2007. Effects of high-fat diet and/or body weight on mammary tumor leptin and apoptosis signaling pathways in MMTV-TGF-alpha mice. Breast Cancer Res. 9(6):R91. 
Origin beta-Actin (A11) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the amino terminal domain of human beta-Actin. 
Product Details Each vial contains 200 g/ml of affinity purified rabbit IgG, beta-Actin (A11) DB070, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Competition Studies A blocking peptide is also available, DB070P, for use in competition studies. Each vial contains 0.100 mg of peptide in 0.5 ml PBS with 0.1% sodium azide and 0.100 mg BSA. 
Immunogen A synthetic peptide mapping to the amino terminal domain of human beta-Actin. 
Specificity Mouse, rat, and human beta-Actin 
Use Western blotting and Immunohistochemistry. Recommended western blotting starting dilution 1:200. 
Storage Store this product at 4 C, do not freeze. The product is stable for one year from the date of shipment. 
References 1.Fuller CL, Braciale VL and Samelson LE. 2003. All roads lead to actin: the intimate relationship between TCR signaling and the cytoskeleton. Immunol Rev. 191:220-236. 2. Giganti A and Friederich E. 2003. The actin cytoskeleton as a therapeutic target: state of the art and future directions. Prog Cell Cycle Res. 5:511-525. 3. Doolittle RF. 1995. The origins and evolution of eukaryotic proteins. Philos Trans Royal Soc London Biol Sci. 349(1329):235-240. 4. Maccioni RB and Cambiazo V. 1995. Role of microtubule-associated proteins in the control of microtubule assembly. Physiol Rev. 75(4):835-864. 5. Schutt CE, Rozycki MD, Myslik JC, Lindberg U. 1995. A discourse on modeling F-actin. J. Struct. Biol. 115:186-198. 6. Barkalow K and Hartwig JH. 1995. Actin cytoskeleton. Setting the pace of cell movement. Curr Biol. 5(9):1000-1002. 

For Technical service please call +1-800-595-1994
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