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EGFR (EGFR1) Antibody DB138 0.100 mg/ml $275.00

Datasheet 

For technical service please call (800) 595 1994
Product Info
Background The EFGR family is a group of four structurally similar tyrosine kinases (EGFR, HER2/neu, ErbB-3, and ErbB-4) that dimerize on binding with a number of ligands, including EGF and transforming growth factor alpha (1&2). The 170 kDa epidermal growth factor receptor binds EGF which activates a tyrosine-specific protein kinase activity intrinsic to the receptor (3&4). Epidermal growth factor receptor overexpression is pronounced in virtually all squamous carcinomas and is also found in > or = 65% of large cell and adenocarcinomas. It is not expressed in situ by small cell lung carcinoma. Overexpression of EGFR is one of the earliest and most consistent abnormalities in bronchial epithelium of high-risk smokers (1). 
*Product Citations* 1.) Weyergang A, Selbo PK, Berg K. 2007. Y1068 phosphorylation is the most sensitive target of disulfonated tetraphenylporphyrin-based photodynamic therapy on epidermal growth factor receptor. Biochem Pharmacol. Jul 15;74(2):226-35. 
Origin EGFR (EGFR1) is a mouse monoclonal IgG2b generated by immunizing mice with the human epidermoid carcinoma line A431. 
Product Details Each vial contains 100 g/ml of mouse monoclonal IgG2b EGFR DB138 (EGFR1), in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Immunogen Human epidermoid carcinoma line A431; epitope mapped between aa 6-273 of human EGFR 
Specificity This antibody is specific for the extracellular region of EGFR. This antibody does not react with erbB-2 (Her-2/Neu), erbB-3 and erbB-4. 
Use EGFR (EGFR1) DB138 can be used in Western blotting, immunoprecipitation, immunohistochemistry (including paraffin-embedded sections), Flow Cytometry / FACS. Western blotting starting dilution 1:200. 
Storage Store this product at 4 C, do not freeze. The product is stable for one year from the date of shipment. 
References 1. Franklin WA, Veve R, Hirsch FR, Helfrich BA, Bunn PA Jr. Epidermal growth factor receptor family in lung cancer and premalignancy. Semin Oncol 2002 Feb;29(1 Suppl 4):3-14.
2. Yarden Y. The EGFR family and its ligands in human cancer, signalling mechanisms and therapeutic opportunities. Eur J Cancer 2001 Sep: 37 Suppl 4: S3-8.
3. Reynolds F.H. Jr., Todaro G.J., Fryling C., and Stephenson J.R. Human transforming growth factors induces tyrosine phosphorylation of EGR receptors. Nature 1981 292: 259-262.
4. Chen W.S., Lazar C.S., Poenie M, Tsien R.Y., Gill G.N., Rosenfeld M.G. Requirement for intrinsic protein tyrosine kinase in the immediate and late actions of the EGF receptor. Nature 1987 328: 820-823.
 

For Technical service please call +1-800-595-1994
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Apoptosis/Tumor Suppressor Genes| Cell Adhesion| Cell Cycle| Controls| Fusion Protein/Epitope Tags| Growth Factors| Membrane Receptors
Protein Kinases| Secondary Antibodies| Signaling Intermediates| Transcription Regulation