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SIRT1 (C19) Antibody DB083 0.200 mg/ml $190.00

Datasheet 
Datasheet 

For technical service please call (800) 595 1994
Product Info
Background The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4 (1). The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53 (1-3). SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras (2&3). The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress (1-3). 
*Product Citations* 1.) Pruitt K, Zinn RL, Ohm JE, McGarvey KM, Kang SH, Watkins DN, Herman JG, Baylin SB. 2006. Inhibition of SIRT1 Reactivates Silenced Cancer Genes without Loss of Promoter DNA Hypermethylation. PLoS Genet. Mar;2(3):e40. Epub 2006 Mar 31.
2.) Ohm JE, McGarvey KM, Yu X, Cheng L, Schuebel KE, Cope L, Mohammad HP, Chen W, Daniel VC, Yu W, Berman DM, Jenuwein T, Pruitt K, Sharkis SJ, Watkins DN, Herman JG, Baylin SB. 2007. A stem cell-like chromatin pattern may predispose tumor suppressor genes to DNA hypermethylation and heritable silencing. Nat Genet. Feb;39(2):237-42. 
Origin SIRT1 (C19) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminal domain of human SIRT1. 
Product Details Each vial contains 200 g/ml of affinity purified rabbit IgG, SIRT1 (C19) DB083, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Competition Studies A blocking peptide is also available, DB083P, for use in competition studies. Each vial contains 0.100 mg of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 mg BSA. 
Form 200 g/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. 
Immunogen Synthetic peptide mapping to the carboxy terminal domain of human SIRT1. 
Specificity SIRT1 (C19) is recommended for use to detect human SIRT1 
Use Western blotting, immunoprecipitation, and CHIP assays. Recommended western blotting starting dilution 1:400. 
Storage Store this product at 4 C, do not freeze. The product is stable for one year from the date of shipment. 
References 1. Smith J. 2002. Human Sir2 and the 'silencing' of p53 activity. Trends Cell Biol. 12(9):404-406.
2. Vaziri H, Dessain SK, Ng Eaton E, Imai SI, Frye RA, Pandita TK, Guarente L, Weinberg RA. 2001. HSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase. Cell 107(2):149-159.
3. Langley E, Pearson M, Faretta M, Bauer UM, Frye RA, Minucci S, Pelicci PG, Kouzarides T. 2002. Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence. EMBO J. 21(10):2383-2396.
 

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