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S-tag (E15)+HRP Antibody DB116 0.200 mg/ml $245.00


For technical service please call (800) 595 1994
Product Info
Background S-tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. Frederick Richards discovered the S-peptide/S-protein system. Ribonuclease A is split by enzyme subtilisin between amino acids 20 and 21 yielding ribonuclease S. Ribonuclease S is composed of the peptide corresponding to the first 20 amino acids, S-peptide, attached to the remainding amino acids 21-124, S-protein. Ribonuclease S has the full enzymatic activity of the parent protein and the two components, S-peptide and S-protein could be separated by reversible denaturation. The separation abolished enzymatic activity, but when the two components were mixed together again, fully active ribonuclease S is formed. The unique property of reconstituting enzymatic activity by the S-tag peptide:S-protein interaction enables sensitive quantitative measurement of any fusion protein by a simple assay. 
Form S-tag (E15)+HRP is provided as an affinity purified rabbit polyclonal antibody conjugated to HRP, raised against a peptide derived from the pancreatic ribonuclease A. 
Product Details Each vial contains 200 g in 0.2ml of affinity purified rabbit IgG, S-tag (E15)+HRP DB116, in a buffer containing 50mM HEPES pH 7.0, 0.1 M soduim chloride, 0.2% BSA and 0.01 % Thimerosal. 
Immunogen Synthetic peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. 
Specificity S-tag (E15)+HRP DB116 recognizes protein that contain the S-tag epitope tag. 
Use S-tag (E15)+HRP DB116 is recommended for use by western blotting, immunoprecipitation, immunohistochemistry, and ELISA. The suggested western blotting starting dilution is at 1:1000. 
Storage Store this product at 4 C, do not freeze. The product is stable for one year from the date of shipment. 

For Technical service please call +1-800-595-1994
Delta Biolabs+1-408-846-6650 • fax:+1-408-846-6645 • Copyright © 2001.Allrights reserved.
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