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S-tag (E15)+FITC Antibody DB117 0.200 mg/0.2ml $300.00


For technical service please call (800) 595 1994
Product Info
Background S-tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. Frederick Richards discovered the S-peptide/S-protein system. Ribonuclease A is split by enzyme subtilisin between amino acids 20 and 21 yielding ribonuclease S. Ribonuclease S is composed of the peptide corresponding to the first 20 amino acids, S-peptide, attached to the remainding amino acids 21-124, S-protein. Ribonuclease S has the full enzymatic activity of the parent protein and the two components, S-peptide and S-protein could be separated by reversible denaturation. The separation abolished enzymatic activity, but when the two components were mixed together again, fully active ribonuclease S is formed. The unique property of reconstituting enzymatic activity by the S-tag peptide:S-protein interaction enables sensitive quantitative measurement of any fusion protein by a simple assay. 
Form S-tag (E15)+FITC is provided as an affinity purified rabbit polyclonal antibody conjugated to HRP, raised against a peptide derived from the pancreatic ribonuclease A. 
Product Details Each vial contains 200 µg in 0.2ml of affinity purified rabbit IgG, S-tag (E15)+FITC DB117, in a buffer containing 50mM HEPES pH 7.0, 0.1 M soduim chloride, 0.2% BSA and 0.01 % Thimerosal and 0.2% gelatin. 
Immunogen Synthetic peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. 
Specificity S-tag (E15)+FITC DB117 recognizes protein that contain the S-tag epitope tag. 
Use S-tag (E15)+FITC DB117 is recommended for use in immunocytochemistry. 
Storage Store this product at 4º C, do not freeze. The product is stable for one year from the date of shipment. 

For Technical service please call +1-800-595-1994
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