Smac/Diablo (C17) Antibody-Delta Biolabs

Product Info
Background	Second mitochondrial activator of caspases or Smac/DIABLO can promote apoptosis by relieving the inhibition of caspases by the IAPs (1&2). The other mitochondrial activator of apoptosis is cytochrome c (1). Smac/DIABLO export from the mitochondria into the cytosol can be triggered by cytotoxic drugs, DNA damage, and the ligation of FAS (CD95) death receptor (3). Once in the cytosol Smac/DIABLO eliminates the inhibitory effects of the IAPs through and amino terminal interaction with the IAP proteins, and thus allow the activation of caspases (2&4). The interaction of Smac/DIABLO?s amino terminus with the IAP proteins has been localized to their BIR domains, for XIAP the key domain is BIR3 (4&5).
*Product Citations*	1.) Kim JS, Eom JI, Cheong JW, Choi AJ, Lee JK, Yang WI, Min YH. 2007. Protein kinase CK2alpha as an unfavorable prognostic marker and novel therapeutic target in acute myeloid leukemia. Clin Cancer Res. Feb 1;13(3):1019-28.
Origin	Smac/DIABLO (C17) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminus of human Smac/DIABLO.
Product Details	Each vial contains 200 �g/ml of affinity purified rabbit IgG Smac/DIABLO (C17) DB045, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Competition Studies	A blocking peptide is also available, DB045P, for use in competition studies. Each vial contains 0.100 mg of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 mg BSA.
Form	Each vial contains 200 �g/ml of affinity purified rabbit IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Immunogen	Synthetic peptide mapping to the carboxy terminus of human Smac/DIABLO.
Specificity	Smac/DIABLO (C17) DB045 reacts with Smac/DIABLO of human origin by western blotting. Western blotting starting dilution 1:200.
Use	Western blot analysis of human Smac/DIABLO expression in HL60 whole cell lysates.
Storage	Store this product at 4� C, do not freeze. The product is stable for one year from the date of shipment.
References	1. Du C., Fang M., Li Y., Li L., Wang X. 2000. Smac, a Mitochondrial Protein that promotes Cytochrome c-Dependent Caspase Activation by Eliminating IAP Inhibition. Cell 102:33-42. 2. Springs S.L., Diavolitsis V.M., Goodhouse J., Mclendon G.L. 2002. The kinetics of translocation of Smac/DIABLO from the mitochondria to the cytosol in HeLa cells. J Biol Chem 3. Adrain C., Creagh E.M., Martin S.J. 2001. Apoptosis-associated release of Smac/DIABLO from mitochondria requires active caspases and is blocked by Bcl-2. EMBO J 20(23):6627-6636. 4. Wu G., Chai J., Suber T.L., Wu J.W., Du C., Wang X., Shi Y. 2000. Structural basis of IAP recognition by Smac/DIABLO. Nature 408(6815):1008-1012. 5. Liu Z., Sun C., Olejniczak E.T., Meadows R.P., Betz S.F., Oost T., Herrmann J., Wu J.C., Fesik S.W. 2000. Structural basis for binding of Smac/DIABLO to the XIAP BIR3 domain. Nature 408(6815):1004-1008.