HDAC1 (C19) Antibody-Delta Biolabs

Product Info
Background	HDAC1, the mammalian homologue of the yeast transcriptional regulator Rpd3p, was first purified using a trapoxin affinity matrix (1). Trapoxin is a cyclotetrapeptide that inhibits histone deacetylation in vivo and also correlates with an increase in the acetylation levels of all four core histones (1&2). These data support a role for HDAC1 as a key histone deacetylase. The function of HDAC1 has been further refined as responsible for the deacetylation of lysine residues on the N-terminal region of the core histones H2A, H2B, H3 and H4 (3&4).
Origin	HDAC1 (C19) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminal domain of human HDAC1.
Product Details	Each vial contains 200 �g/ml of affinity purified rabbit IgG, HDAC1 (C19) DB071, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Competition Studies	A blocking peptide is also available, DB071P, for use in competition studies. Each vial contains 0.100 mg of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 mg BSA.
Form	200 �g/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Immunogen	Synthetic peptide mapping to the carboxy terminal domain of human HDAC1.
Specificity	HDAC1 (C19) is recommended to detect mouse, rat, and human HDAC1.
Use	Western blotting. Recommended western blotting starting dilution 1:200.
Storage	Store this product at 4� C, do not freeze. The product is stable for one year from the date of shipment.
References	1. Taunton J, Hassig CA, Schreiber SL. 1996. A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. Science 272:408-411. 2. Hassig CA, Tong JK, Fleischer TC, Owa T, Grable PG, Ayer DE, Schreiber SL. 1998. A role for histone deacetylase activity in HDAC1-mediated transcriptional repression. PNAS 95(7):3519-3524. 3. Lopez-Rodas G, Brosch G, Georgieva EI, Sendra R, Franco L, Loidl P. 1993. Histone deacetylase. A key enzyme for the binding of regulatory proteins to chromatin. FEBS lett 317(3):175-180. 4. Johnson CA, White DA, Lavender JS, O?Neill LP, Turner BM. 2002. Human class I histone deacetylase complexes show enhanced catalytic activity in the presence of ATP and co-immunoprecipitated with ATP-dependent charperone protein Hsp-70.