GFP (FL3) Antibody-Delta Biolabs

Product Info
Background	Green fluorescent protein was originally cloned from the cnidarian, Aequorea victoria. This exceptional protein absorbs blue light (maximally at 395 nm) and emits green light (peak at 509) without the requirement of exogenous substrates and cofactors (1). These unique qualities allow GFP to be used to monitor gene expression and protein localization in vivo. Several mutant forms of GFP have been developed which fluoresce more intensely and have shifted excitation maxima when compared to the wild type GFP, making them useful for FACS, fluorescence microscopy, and double-labeling applications (2,3).
Origin	Rabbits were immunized with highly purified green-fluorescent protein (GFP) isolated directly from Aequorea victoria. Antibodies were affinity purified using the native GFP immobilized on a solid phase.
Product Details	Each vial contains 100 �g/ml of affinity purified rabbit IgG, GFP DB088 (FL3), in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Form	100 �g/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Immunogen	Highly purified green-fluorescent protein (GFP) isolated directly from Aequorea victoria.
Specificity	DB088 GFP (FL3) recognizes all variants of recombinant Aequorea green-fluorescent protein.
Use	ELISA, Western blotting, immunoprecitation, and immunhistochemistry. Suggested working dilutions: Elisa: 1:10,000-1:100,000, western blotting: 1:1,000-1:5,000.
Storage	Store this product at 4� C, do not freeze. The product is stable for one year from the date of shipment.
References	1. Chalfie M, Tu Y., Euskirchen G., Ward W.W., Prasher D.C. 1994. Green Fluorescent Protein as a Marker for Gene Expression. Science 263: 802-805. 2. Cormack B.P., Valdivia R.H., and Falkow S. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38. 3. Rizzuto R., Brini M., De Giorgi F., Rossi R., Heim R., Tsien R.Y., and Pozzan T. 1996. Double labelling of the subcellular structures with organelle-targeted GFP mutants in vivo. Curr.Biol. 6:183-188.