beta-Actin (A11) Antibody-Delta Biolabs

Product Info
Background	All eukaryotic cell types express actin. The regulation of the actin cytoskeleton is tightly linked to vital biological properties such as polarity, motility, cell-cell contact, exocytosis and proliferation (1&2). While lower eukaryotes such as yeast, have only one actin gene; higher eukaryotes have several isoforms encoded by a family of genes (3&4). At least six types of actin are present in mammalian tissues and fall into three classes (5). Alpha-actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues (6).
*Product Citations*	1.) Hoffrogge R, Mikkat S, Scharf C, Beyer S, Christoph H, Pahnke J, Mix E, Berth M, Uhrmacher A, Zubrzycki IZ, Miljan E, Volker U, Rolfs A. 2006. 2-DE proteome analysis of a proliferating and differentiating human neuronal stem cell line (ReNcell VM). Proteomics. Mar;6(6):1833-47. 2.) Dogan S, Hu X, Zhang Y, Maihle NJ, Grande JP, Cleary MP. 2007. Effects of high-fat diet and/or body weight on mammary tumor leptin and apoptosis signaling pathways in MMTV-TGF-alpha mice. Breast Cancer Res. 9(6):R91.
Origin	beta-Actin (A11) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the amino terminal domain of human beta-Actin.
Product Details	Each vial contains 200 �g/ml of affinity purified rabbit IgG, beta-Actin (A11) DB070, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Competition Studies	A blocking peptide is also available, DB070P, for use in competition studies. Each vial contains 0.100 mg of peptide in 0.5 ml PBS with 0.1% sodium azide and 0.100 mg BSA.
Immunogen	A synthetic peptide mapping to the amino terminal domain of human beta-Actin.
Specificity	Mouse, rat, and human beta-Actin
Use	Western blotting and Immunohistochemistry. Recommended western blotting starting dilution 1:200.
Storage	Store this product at 4� C, do not freeze. The product is stable for one year from the date of shipment.
References	1.Fuller CL, Braciale VL and Samelson LE. 2003. All roads lead to actin: the intimate relationship between TCR signaling and the cytoskeleton. Immunol Rev. 191:220-236. 2. Giganti A and Friederich E. 2003. The actin cytoskeleton as a therapeutic target: state of the art and future directions. Prog Cell Cycle Res. 5:511-525. 3. Doolittle RF. 1995. The origins and evolution of eukaryotic proteins. Philos Trans Royal Soc London Biol Sci. 349(1329):235-240. 4. Maccioni RB and Cambiazo V. 1995. Role of microtubule-associated proteins in the control of microtubule assembly. Physiol Rev. 75(4):835-864. 5. Schutt CE, Rozycki MD, Myslik JC, Lindberg U. 1995. A discourse on modeling F-actin. J. Struct. Biol. 115:186-198. 6. Barkalow K and Hartwig JH. 1995. Actin cytoskeleton. Setting the pace of cell movement. Curr Biol. 5(9):1000-1002.