Cdk4 (C22) Antibody-Delta Biolabs

Product Info
Background	Cyclin dependent kinases are key regulators of the progression of the cell cycle. Early in the cell cycle Cdk2, Cdk4, Cdk6 and their associated cyclins regulate the G1 to S phase transition (1, 2). Cdk2 plays a key role in the G1/S and S/G2 transitions through its associations with cyclin D1, cyclin D2, cyclin D3, cyclin E and cyclin A. Cdk4 also forms complexes with the D type cyclins, and is thought to regulate cell growth through the G1 phase of the cell cycle (3-6). The late stages of the cell cycle are regulated by another cyclin dependent kinase, Cdc2 p34. This kinase exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34-cyclin B complex that is required for the G2 to M phase transition (7,8).
*Product Citations*	1.) Pawar SA, Szentirmay MN, Hermeking H, Sawadogo M. 2004. Evidence for a cancer-specific switch at the CDK4 promoter with loss of control by both USF and c-Myc. Oncogene 12;23(36):6125-35. 2.) Kim TH, Oh S, Kim SS. 2005. Recombinant human prothrombin kringle-2 induces bovine capillary endothelial cell cycle arrest at G0-G1 phase through inhibition of cyclin D1/CDK4 complex: modulation of reactive oxygen species generation and up-regulation of cyclin-dependent kinase inhibitors. Angiogenesis. 8(4):307-14.
Origin	Cdk4 (C22) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminus of mouse Cdk4.
Product Details	Each vial contains 200 �g/ml of affinity purified rabbit IgG, Cdk4 (C22) DB011, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Competition Studies	A blocking peptide is also available, DB011P, for use in competition studies. Each vial contains 0,100 mg of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 mg BSA.
Form	200 �g/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Immunogen	Synthetic peptide mapping to the carboxy terminus of mouse Cdk4.
Specificity	Cdk4 (C22) DB011 reacts with Cdk4 of mouse, rat, and human origin
Use	Western blotting, immunoprecipitation and immunohistochemistry. Western blotting starting dilution: 1:200.
Storage	Store this product at 4� C, do not freeze. The product is stable for one year from the date of shipment.
References	1. Ekholm SV, Reed SI. 2000. Regulation of G(1) cyclin-dependent kinases in the mammalian cell cycle. Curr Opin Cell Biology 12(6): 676-684 2. Morisaki H, Ando A, Nagata Y, Pereira-Smith O, Smith JR, Ikeda K, Nakanishi M. 1999. Complex mechanisms underlying impaired activation of Cdk4 and Cdk2 in replicative senescence: roles of p16, p21, and cyclin D1. Exp Cell Research 253(2): 503-510. 3. Tong W, Pollard JW. 1999. Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice. Mol Cell Biology 19(3): 2251-64. 4. Sweeney KJ, Sarcevic B, Sutherland RL, Musgrove EA. 1997. Cyclin D2 activates Cdk2 in preference to Cdk4 in human breast epithelial cells. Oncogene 14(11): 1329-1340. 5. Chu CY, Lim RW. 2000. Involvement of p27(kip1) and cyclin D3 in the regulation of cdk2 activity during skeletal muscle differentiation. Biochimica Biophysica Acta 1497(2): 175-185. 6. Kishimoto T, Okumura E. In vivo regulation of the entry into M-phase: initial activation and nuclear translocation of cyclin B/Cdc2. 1997. Prog Cell Cycle Res 3:241-249. 7. Morla AO, Draetta G, Beach D, Wang JY. 1989. Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis. Cell 58(1): 193-203. 8. O?Connor PM, Ferris DK, Pagano M, Draetta G, Pines J, Hunter T, Longo DL, Kohn KW. 1993. G2 delay induced by nitrogen mustard in human cells affects cyclin A/cdk2 and cyclin B1/cdc2-kinase complexes differently. J Biological Chemistry 268(11): 8298-8308.