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Product Info
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Background |
The 6x histidine recombinant protein tag is made up of 6 histidine residues attached to either the amino or carboxy terminus of the protein being expressed. The recombinant protein can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6x histidine in techniques such as western blotting and immunoprecipitation.
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*Product Citations* |
1.) Wu B, Wilmouth RC. 2008. Proteomics analysis of immunoprecipitated proteins associated with the oncogenic kinase cot. Mol Cells. Feb 29;25(1):43-9.
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Origin |
His (H20) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide containing the 6x histidine tag.
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Product Details |
Each vial contains 200 µg/ml of affinity purified rabbit IgG, His (H20) DB063, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
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Competition Studies |
A blocking peptide is also available, DB063P, for use in competition studies. Each vial contains 0.100 mg of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 mg BSA.
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Form |
200 µg/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
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Immunogen |
Synthetic a peptide containing the 6x histidine tag.
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Specificity |
His (H20) DB063 detects proteins contain the 6X His tag. His (H20) is recommended for use by western blotting and immunoprecipitation. The suggested western blotting starting dilution is at 1:200.
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Use |
Western blot analysis of polyhistidine tagged recombinant HspBP1 at 0.1 µg (A) and 0.01 µg (B).
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Storage |
Store this product at 4º C, do not freeze. The product is stable for one year from the date of shipment
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